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การเหนี่ยวนำการเปลี่ยนแปลงไปเป็นเซลล์ประสาทของเซลล์ต้นกำเนิดปลายรากฟันมนุษย์โดยสารเรสเวอราทรอลได้ผลดีในการเพิ่มปริมาณเซลล์ประสาทของเซลล์ต้นกำเนิดเพื่อจะใช้ประโยชน์เป็นเซลล์บำบัดในการรักษาโรคความผิดปกติระบบประสาทส่วนกลาง สารนี้ช่วยเพิ่มปริมาณเซลล์ตั้งต้นเซลล์ประสาทสำหรับการปลูกถ่ายเซลล์ประสาท เพื่อประยุกต์ใช้ในการรักษาโรคต่างที่เกี่ยวกับระบบประสาท เช่น Sensorineural hearing loss (SNHL), Parkinson ‘s disease, Alzheimer’s disease
Abstract
Introduction
Stem cell transplantation of exogenous neural progenitor cells (NPCs) derived from mesenchymal stem cells (MSCs) has emerged as a promising approach for neurodegenerative disease. Human stem cells from apical papilla (hSCAPs) are derived from migratory neural crest stem cells and exhibit a potential of neuronal differentiation. However, their neuronal differentiation is low and unpredictable. Resveratrol has been described as a sirtuin 1 (SIRT1) activator which plays an important role in enhancing neuronal differentiation. In this study, we investigate the potential of resveratrol as an enhancer on neuronal differentiation through NPCs induction of hSCAPs.
Methods
Stem cells were isolated from human apical papilla and characterized as MSCs. The cellular toxicity of resveratrol treatment to the characterized hSCAPs was investigated by MTT assay. The non-cellular toxicity concentrations of resveratrol were assessed with various pre-treatment times to select the optimal condition that highly expressed the neural progenitor gene, NES. Consequently, the optimal condition of resveratrol pre-treatment was synergistically performed with a neuronal induction medium to trigger neuronal differentiation. The differentiated cells were visualized, the genes profiling was quantified, and the percentage of neuronal differentiation was calculated. Moreover, the intracellular calcium oscillation was demonstrated.
Results
The cellular toxicity of resveratrol was not observed for up to 50 μM for 12 h. Interestingly, hSCAPs pre-treated with 10 μM resveratrol for 12 h (RSV-hSCAPs) significantly expressed NES, which is determined as the optimal condition. Under neuronal induction, both of hSCAPs and RSV-hSCAPs were differentiated (d-hSCAPs and RSV-d-hSCAPs) as they exhibited neuronal-like appearances with Nissl substance staining. The highest expression of NES and SOX1 was observed in RSV-d-hSCAPs. Additionally, the percentage of neuronal differentiation of RSV-d-hSCAPs was significantly higher than d-hSCAPs for 4 times. Importantly, the neuronal-like cells exhibited slightly increasing pattern of calcium intensity.
Conclusion
This study demonstrated that pre-treatment of resveratrol strongly induces neural progenitor marker gene expression which synergistically enhances neural progenitor-like cells’ induction with neuronal induction medium.
KEYWORDS: Resveratrol, Neural progenitor-like cells, Human stem cells from apical papilla
Citation: Songsaad, A., Gonmanee, T., Ruangsawasdi, N. et al. Potential of resveratrol in enrichment of neural progenitor-like cell induction of human stem cells from apical papilla. Stem Cell Res Ther11, 542 (2020).
https://doi.org/10.1186/s13287-020-02069-9
RELATED SDGs: 3. GOOD HEALTH AND WELL-BEING
ผู้ให้ข้อมูล: รองศาสตราจารย์ ดร.เจริญศรี ธนบุญสมบัติ
ชื่ออาจารย์ที่ทำวิจัย: รองศาสตราจารย์ ดร.เจริญศรี ธนบุญสมบัติ
ชื่อนักศึกษาที่ทำวิจัย: นายอนุพงศ์ สงค์สะอาด
แหล่งทุนวิจัย: Science Achievement Scholarship of Thailand (SAST), partially supported by Central Instrument Facilities (CIF) Faculty of Science, Mahidol University, Thailand.
Tags: Human stem cells from apical papilla, Neural progenitor-like cells, Resveratrol
