Abstract
The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles. However, little is known about the host surface molecules to which MrNV attach to initiate infection. Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills. We established that MrNV in their viruslike particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F. The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins. Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans. Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections
KEYWORDS: fucosylation, glycans, Macrobrachium rosenbergii, nodavirus, virus-like particles
Citation: Somrit M, Yu S-Y, Le Pendu J, et al.Macrobrachium rosenbergii nodavirus virus-like particles attachto fucosylated glycans in the gills of the giant freshwaterprawn. Cellular Microbiology. 2020;22:e13258. https://doi.org/10.1111/cmi.13258
RELATED SDGs: 2. ZERO HUNGER
ผู้ให้ข้อมูล: อาจารย์ ดร.มนสิชา สมฤทธิ์
ชื่ออาจารย์ที่ทำวิจัย: รองศาสตราจารย์ ดร.วัฒนา วีรชาติยานุกูล, อาจารย์ ดร.มนสิชา สมฤทธิ์
แหล่งทุนวิจัย: Thailand Research Fund, Grant/ Award Number: MRG6180055-Monsicha Somrit, Research Support Grant of the Faculty of Science, Mahidol University, Srinakharinwirot University research grant: 4, Grant/Award Number: 93/2559; Srinakharinwirot University government grant: 013/2561,Franco-Thai Junior Fellowship 2018-2019,
Tags: fucosylation, glycans, Macrobrachium rosenbergii, nodavirus, virus-like particles
